Ling Yuan

Ling Yuan

Professor

lyuan3@uky.edu
Phone (859) 257-4806
Fax (859) 323-1077
Yuan Lab Home Page

University of Kentucky, PSS
KTRDC
1401 University Drive (Office:200L) Lexington KY 40546-0236

Professional Profile

Specialty
Gene transcriptional regulation, protein engineering, directed evolution, metabolic pathway engineering

My research interests are centered in two main areas:

  1. mechanistic study of transcription factors (TFs) for plant metabolic pathway regulation, and
  2. improvement of bioenergy production through engineering tobacco plants to produce high-density biofuels and engineering enzymes for novel functions in biomass conversion.

A new research focus in my lab is the modification of leaf characteristics and surface chemistry. The research in my lab addresses some of the key issues in molecular biology and protein biochemistry and contributes to the development of new-crop opportunities and bioenergy, two important areas in the agricultural economies of Kentucky and the nation. My current research is supported by the US Department of Agriculture, the Department of Energy, The Kentucky Office of Energy Policy and Kentucky Tobacco Research and Development Center, University of Kentucky.    More....

Education

  • 1989, Postdoctoral fellow, Univeristy of California, San Francisco, Pharmaceutical Chemistry
  • 1989, Ph.D., University of Texas at Austin, Biochemistry and molecular biology
  • 1983, B.S. Jinan University, Guangzhou, China, Biology

RECENT PUBLICATIONS

Hongxia Wang, JunYang, Min Zhang, Weijuan Fan, Nurit Firo, Sitakanta Pattanaik, Ling Yuan,, and Peng Zhang 2015 Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development. Scientific Reports. 6:18645  Abstract

Singh SK, Wu Y, Ghosh JS, Pattanaik S, Fisher C, Wang Y, Lawson D, Yuan L 2015 RNA-sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-shoot Control Chemicals. Scientific Reports. 5:18148. Abstract

Chen J, Xu Y, Wei G, Liao S, Zhang Y, Huang W, Yuan L and Wang Y 2015 Chemotypic and genetic diversity in Epimedium sagittatum from different geographical regions of China.Phytochemistry. 116:180-7 Abstract

Jia X, Shen J, Liu H, Li F, Ding N, Gao C, Pattanaik S, Patra B, Li R , and Yuan L. 2015 Small tandem target mimic-mediated blockage of microRNA858 induces anthocyanin accumulation in tomato. Planta.  Abstract

Schluttenhofer CM and Yuan L 2015 Regulation of Specialized Metabolism by WRKY Transcription Factors.Plant physiology. 167(2):295-306. Abstract

Yuan L and Grotewold E 2015 Metabolic engineering to enhance the value of plants as green factories.Metabolic Engineering.27:83-91. Abstract

Schluttenhofer CM, Pattanaik S, Patra B, and Yuan L 2014 Analyses of Catharanthus roseus and Arabidopsis thaliana WRKY transcription factors reveal involvement in jasmonate signaling.BMC Genomics 15:502. Abstract

Pattanaik S, Patra B, Singh SK and Yuan L 2014 An overview of the gene regulatory network controlling trichome development in the model plant, Arabidopsis.Frontier in Plant Cell Biology 5:259. Abstract

Singh SK, Heng C, Braker JD, Chan VJ, Lee CC, Jordan DB, Yuan L and Wagschal K. 2014 Directed evolution of GH43 beta-xylosidase XylBH43 thermal stability and L186 saturation mutagenesis.Journal of Industrial Microbiology and Biotechnology 41(3):489-98. Abstract

Patra B, Schluttenhofer CM, Wu Y, Pattanaik S and Yuan L 2013 Transcriptional regulation of secondary metabolism in plants. BBA-Gene Regulatory Mechanisms 1829(11):1236-4. Abstract

Yang Z , Patra B, Li R, Pattanaik S and Yuan L 2013 Promoter analysis reveals cis-regulatory motifs associated with the expression of the WRKY transcription factor CrWRKY1 in Catharanthus roseus. Planta.238 (6)1039-1049 Abstract

Huang W, Sun W, Lv H, Luo M, Zeng S, Pattanaik S, Yuan L and Wang Y 2013 A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway. Plos One 8(8): e70778. Abstract

Patra B, Pattanaik S and Yuan L 2013. Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26Sproteasome system. Plant Signaling and Behavior.8:e25901 Abstract

Patra B, Pattanaik S and Yuan L 2013. Ubiquitin protein ligase 3 mediates the proteasomal degradation of GLABROUS 3 and ENHANCER OF GLABROUS 3, regulators of trichome development and flavonoid biosynthesis in Arabidopsis. The Plant Journal 74(3):435-47. Abstract

Kong Q, Pattanaik S, Feller A, Werkman JR , Chai C, Wang Y, Grotewold E and Yuan L 2012 Regulatory switch enforced by basic helix-loop-helix and ACT-domain mediated dimerizations of the maize transcription factor R. Proceeding of National Academy of Sciences U.S.A. 109: E2091-2097.  Abstract

Bai Y, Pattanaik S, Patra B, Werkman JR, Xie CH and Yuan L 2011 Flavonoid-related basic helixloop- helix regulators, NtAn1a and NtAn1b, of tobacco have originated from two ancestors and are functionally active. Planta 234:363-375.  Abstract

Jordan DB, Wagscha K, Fan ZM, Yuan L, Braker JD and Heng C 2011 Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145. Journal of Industrial Microbiology and Biotechnology 38:1821-1835.  Abstract

Suttipanta N, Pattanaik S, Kulshrestha M, Patra B, Singh SK and Yuan L 2011 The Transcription Factor CrWRKY1 Positively Regulates Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus. Plant Physiology. 157: 2081-2093.  Abstract

Fan ZM and Yuan L 2010 Production of Multifunctional Chimeric Enzymes in Plants: a promising Approach for Degrading Plant Cell Wall from Within. Plant Biotechnology Journal. 8:308-315.  Abstract

Fan ZM, Yuan L, Jordan DB, Wagschal K, Heng C and Braker JD 2010 Engineering Lower Inhibitor Affinities in Beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113. Abstract

Pattanaik S, Kong Q, Zaitlin D, Werkman JR, Xie CH, Patra, B, and Yuan L, 2010 Isolation and functional characterization of a floral tissue-specific R2R3 MYB regulator from tobacco. Planta. 231(5):1061-1076. Abstract

Fan ZM, Werkman JR, and Yuan L 2009 Engineering a multifunctional hemicellulase. Biotechnology Letters 31:751-757. Abstract

Fan ZM, Yuan L and Chatterjee R 2009 Increased Hydrogen Production by Genetic Engineering of Escherichia coli. PLoS ONE 4(2): e4432.  Abstract

Fan ZM, Wagschal K, Chen W, Montross MD, Lee CC and Yuan L 2009 Multimeric Hemicellulases Facilitate Biomass Conversion. Applied and Environmental Microbiology. 75: 1754-1757. Abstract

BOOKS AND BOOK CHAPTERS

Yuan L and Perry S (Editors) 2011 Plant Transcription Factors: Methods and Protocols. Methods in Molecular Biology. Vol. 754. Abstract

Pattanaik S, Werkman JR and Yuan L 2011 Biomolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts. pp. 185-193. IN: L. Yuan, and S. Perry, eds. Plant Transcription Factors: Methods and Protocols. vol. 754, Methods in Molecular Biology. Humana Press, New York. Abstract

Werkman JR, Pattanaik S, and Yuan L 2011 Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters. Chapter 19, pp. 325-342. IN: L. Yuan and S.E. Perry, eds. Plant Transcription Factors: Methods and Protocols, vol. 54. In Methods in Molecular Biology (J.M. Walker, series ed.), Humana Press, New York.  Abstract

Pattanaik S, Werkman JR, Kong Q and Yuan L 2010 Site-directed mutagenesis and saturation mutagenesis for the functional study of transcription factors involved in plant secondary metabolite biosynthesis. pp. 47-57. IN: Metabolic Engineering of Plant Secondary Pathways, vol. 643 in Methods in Molecular Biology, Springer, New York. Abstract

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