Art Hunt

Arthur Hunt

Professor

aghunt00@uky.edu
Phone (859) 218-0776
Fax (859) 257-7125
Hunt Lab Web Site

301A Plant Science Building,
1405 Veterans Drive, Lexington KY 40546-0312

AREAS OF INTEREST

My lab studies RNA processing in plants, with an emphasis on mRNA 3' end formation and polyadenylation.  The approaches we take are multi-faceted, and include genetic, molecular, and biochemical ones.  The specific projects being pursued revolve around questions pertaining to mehanisms of mRNA 3' end formation, and to the connections between this process and regulatory events.  Extensive use is made of the model organisms Arabidopsis thaliana, Escherichia coli and Saccharomyces cerevisiae.

PROFESSIONAL PROFILE

  • 1996 - Present Professor, Department of Agronomy (now Plant and Soil Sciences), University of Kentucky
  • 1990 - 1996 Associate Professor, Department of Agronomy, University of Kentucky
  • 1985 - 1990 Assistant Professor, Department of Agronomy, University of Kentucky
  • 1982 - 1985 Postdoctoral Scientist, The Rockefeller University

EDUCATION

  • 1982 Ph.D., Biochemistry, Brandeis University
  • 1976 B.S., Biological Sciences, University of Lowell

RECENT PUBLICATIONS

Ma, L., Pati, P. K., Liu, M., Li, Q. Q., and Hunt, A. G. (2014) High throughput determination of polyadenylation sites in plants. Methods 67, 74-83.

Wu, X., Gaffney, B, Li, Q. Q., and Hunt, A. G. (2014) Genome-wide determination of poly(A) sites in Medicago truncatula: evolutionary conservation of alternative poly(A) site choice. BMC Genomics 15, 615.

Liu, M., Xu, R., Merrill, C., Von Lanken, C., Hunt, A. G., and Li, Q. Q. (2014) Integration of developmental and hormonal signals via a  polyadenylation factor in Arabidopsis. PloS ONE, 9(12): e115779.

Chakrabarti, M., Dinkins, R. D., and Hunt, A. G. (2015) De novo transcriptome assembly and dynamic spatial gene expression analysis in  red clover (Trifolium pratense). The Plant Genome, accepted for publication (Dec. 10, 2015).

Bell, S. S., Brown, A., Chen, S., and Hunt, A. G. (2015) Experimental genome-wide determination of RNA polyadenylation in Chlamydomonas reinhardtii. PLoS ONE, accepted for publication (Dec. 15, 2015).

RECENT INVITED REVIEWS AND BOOK CHAPTERS

Addepalli, B., Rao, S., and Hunt, A. G. (2015) Phage Display Library Screening for Identification of Interacting Protein Partners. in Polyadenylation in Plants – Methods and Protocols, Methods in Molecular Biology, vol. 1255. A. G. Hunt and Q. Q. Li, eds. Springer. ISBN 978-1-4939-2174-4. pp. 147-158.

Pati, P. K., Ma., L., and Hunt, A. G. (2015) Genome-wide determination of poly(A) site choice in plants. in Polyadenylation in Plants – Methods and Protocols, Methods in Molecular Biology, vol. 1255. A. G. Hunt and Q. Q. Li, eds. Springer. ISBN 978-1-4939-2174-4. pp. 159-174.

Von Lanken, C. and Hunt, A. G. (2015) Transient expression using Agroinfiltration to study polyadenylation in plants. in Polyadenylation in  Plants – Methods and Protocols, Methods in Molecular Biology, vol. 1255. A. G. Hunt and Q. Q. Li, eds. Springer. ISBN 978-1-4939-2174-4. pp. 127-133.

Ma, L. and Hunt, A. G. (2015) A 3’ RACE protocol to confirm polyadenylation sites. in Polyadenylation in Plants – Methods and Protocols, Methods in Molecular Biology, vol.1255. A. G. Hunt and Q. Q. Li, eds. Springer. ISBN 978-1-4939-2174-4. pp. 135-144.

Hunt, A. G. (2015) A rapid, simple, and inexpensive method for the preparation of strandspecific RNA-Seq libraries. in Polyadenylation in Plants – Methods and Protocols, Methods in Molecular Biology, vol. 1255. A. G. Hunt and Q. Q. Li, eds. Springer. ISBN 978-1-4939-2174-4. pp. 195-208.

Chakrabarti, M. and Hunt, A. G. (2015) CPSF30 at the Interface of Alternative Polyadenylation and Cellular Signaling in Plants. Biomolecules 5(2), 1151-1168.

Entire Curriculum Vitae