Lucas Araujo

Lucas Araujo

lucas.araujo@uky.edu

410 Plant Science Building,
1405 Veterans Drive, Lexington KY 40546-0312

ADVISOR

Dr. Michael Barrett

RESEARCH ABSTRACT (From 2017 IPSS Symposium)

"Characterization of 2,4-D tolerance in a novel variety of red clover (Trifolium pratense)"

Incorporation of a legume, such as red clover (Trifolium pratense), into grass-based pasture systems offers many benefits. However, available red clover lines are highly susceptible to herbicides, in particular 2,4-D (2,4-dichlorophenoxyacetic acid), which has been widely used for broadleaf weed management in pastures. A novel red clover line, UK2014, was developed at University of Kentucky through conventional breeding and expresses higher tolerance to 2,4-D than varieties commonly used by Kentucky’s forage producers. Adopting this new tolerant line would broaden weed management options in a legume-grass mixed pasture. The main objective of this study is to assess the field performance of UK2014 and evaluate its tolerance level to 2,4-D in Kentucky’s environment.
Field studies were stablished in 2016 and 2017 to assess UK2014 in comparison with Kenland. Kenland is one of UK2014 parental lines, highly adapted to Kentucky’s environment, but very susceptible to 2,4-D. Plots were treated with 2,4-D at three different seasons, and evaluated for visual herbicide injury and yield. Preliminary results display an increased tolerance in the UK2014 line and a higher regrowth capability after harvest when compared to Kenland. Complementary studies in UK2014 line include metabolism and translocation assays to determine the relationship of the tolerance acquired by this line with the level of metabolized herbicide in the plant. Moreover, a whole transcriptome analysis is in progress at the University of Kentucky to assess differentially expressed genes in both UK2014 and Kenland, following 2,4-D treatment.